8JZV

RPA70N-ETAA1 fusion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-27
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	P 32 2 1
Unit cell lengths	50.209, 50.209, 94.526
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.500 - 1.500
R-factor	0.1846
Rwork	0.184
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7xv4
RMSD bond length	0.012
RMSD bond angle	1.261
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.20rc1_4392: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.500	1.540
High resolution limit [Å]	1.500	1.500
Rmerge	0.057	0.221
Rpim		0.079
Number of reflections	42672	2702
<I/σ(I)>	26.24
Completeness [%]	100.0
Redundancy	9.4
CC(1/2)	0.997	0.981

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	PEG 3350, Ammonium chloride