8JZK

Crystal structure of glutathione S-transferase Tau7 from Salix lindleyana in complex with GSH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-03-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979183
Spacegroup name	C 2 2 21
Unit cell lengths	60.727, 87.931, 113.973
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.340 - 2.550
R-factor	0.19172701895
Rwork	0.188
R-free	0.25835
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.146
Data reduction software	xia2
Data scaling software	xia2
Refinement software	PHENIX (1.9_1692+SVN)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.340	2.550
High resolution limit [Å]	2.550	2.550
Rmeas	0.233
Rpim	0.071
Number of reflections	10186	724
<I/σ(I)>	9.7
Completeness [%]	99.3
Redundancy	5.6
CC(1/2)	0.978	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289.15	0.1M BIS-TRIS propane pH 9.0, 8% w/v Polyethylene glycol 20000