8JYY

Crystal structure of the gasdermin-like protein RCD-1-2 from Neurospora crassa

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-01
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	65.550, 146.706, 126.076
Unit cell angles	90.00, 104.57, 90.00

Refinement procedure

Resolution	48.390 - 2.650
R-factor	0.22
Rwork	0.219
R-free	0.26440
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.456
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.900	2.710
High resolution limit [Å]	2.650	2.650
Rmerge	0.108	0.797
Rmeas	0.120	0.882
Rpim	0.052	0.374
Number of reflections	66913	4503
<I/σ(I)>	12	2.2
Completeness [%]	99.9	100
Redundancy	5.3	5.5
CC(1/2)	0.996	0.773

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	291	0.8 M succinic acid pH 7.0