8JYY
Crystal structure of the gasdermin-like protein RCD-1-2 from Neurospora crassa
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL45XU |
Synchrotron site | SPring-8 |
Beamline | BL45XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-08-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.550, 146.706, 126.076 |
Unit cell angles | 90.00, 104.57, 90.00 |
Refinement procedure
Resolution | 48.390 - 2.650 |
R-factor | 0.22 |
Rwork | 0.219 |
R-free | 0.26440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.456 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.900 | 2.710 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.108 | 0.797 |
Rmeas | 0.120 | 0.882 |
Rpim | 0.052 | 0.374 |
Number of reflections | 66913 | 4503 |
<I/σ(I)> | 12 | 2.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 5.3 | 5.5 |
CC(1/2) | 0.996 | 0.773 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 0.8 M succinic acid pH 7.0 |