8JYA
Crystal Structure of Intracellular B30.2 Domain of VpBTN3 in Complex with IPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-03-17 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34138 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 44.354, 67.934, 134.692 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.170 - 1.500 |
| R-factor | 0.1794 |
| Rwork | 0.178 |
| R-free | 0.21420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zxk |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.745 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.170 | 1.520 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.053 | 0.322 |
| Number of reflections | 65817 | 2515 |
| <I/σ(I)> | 22.53 | |
| Completeness [%] | 99.4 | |
| Redundancy | 10.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M Bis Tris pH 5.5, 2.0 M ammonium sulfate |
