8JY9
Crystal Structure of Intracellular B30.2 Domain of VpBTN3 in Complex with HMBPP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | LIQUID ANODE |
| Source details | BRUKER METALJET |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-28 |
| Detector | Bruker PHOTON III |
| Wavelength(s) | 1.34138 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 43.928, 67.462, 134.597 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.390 - 1.830 |
| R-factor | 0.1901 |
| Rwork | 0.188 |
| R-free | 0.24180 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5zxk |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.554 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.390 | 1.860 |
| High resolution limit [Å] | 1.830 | 1.830 |
| Rmerge | 0.123 | 0.481 |
| Number of reflections | 36047 | 1632 |
| <I/σ(I)> | 13.45 | |
| Completeness [%] | 99.6 | |
| Redundancy | 10.07 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M Bis Tris pH 5.5, 2.0 M ammonium sulfate |
