8JX8
rat megalin head
EM specimen
Vitrification | Yes |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.3 |
Software | Topaz,PHENIX,RELION,SerialEM,Coot,ISOLDE |
Symmetry | C1 |
CTF correction | PHASE FLIPPING ONLY |