8JWS

ePHD domain of PHD Finger Protein 7 (PHF7)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-24
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97957
Spacegroup name	P 21 21 21
Unit cell lengths	37.014, 59.145, 170.396
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.010 - 2.000
R-factor	0.1986
Rwork	0.197
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4nn2
RMSD bond length	0.012
RMSD bond angle	1.234
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.010	2.072
High resolution limit [Å]	2.000	2.000
Rmerge	0.095	0.524
Rmeas	0.113	0.627
Rpim	0.061	0.339
Number of reflections	25458	2513
<I/σ(I)>	8.33	2.8
Completeness [%]	97.0	98.01
Redundancy	3.2	3.2
CC(1/2)	0.994	0.712

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.7	277	PEG 3350, LiCl, Bicine