8JW3
The crystal structure of the viral terpene synthase from Orpheovirus IHUMI-LCC2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2022-03-01 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.98 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.442, 46.763, 69.210 |
| Unit cell angles | 90.00, 116.34, 90.00 |
Refinement procedure
| Resolution | 42.480 - 1.450 |
| R-factor | 0.1776 |
| Rwork | 0.177 |
| R-free | 0.19290 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Se substituted OILTS |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.838 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.480 | 1.470 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rpim | 0.023 | 0.366 |
| Number of reflections | 57694 | 2840 |
| <I/σ(I)> | 18.7 | 2 |
| Completeness [%] | 99.8 | |
| Redundancy | 6.7 | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 277 | 0.1 M Citric acid buffer pH 4.5, 1.3 M Ammonium sulfate |
