8JVZ

Crystal structure of the circular-permutated protein mkaL2_v1_SH3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-12
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	90.182, 28.280, 38.768
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.768 - 1.892
R-factor	0.2205
Rwork	0.219
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.013
RMSD bond angle	1.478
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.892	1.892
Number of reflections	15179	2445
<I/σ(I)>	9.97
Completeness [%]	99.2
Redundancy	3.4
CC(1/2)	0.990	0.700

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	30% 2-propanol, 100mM Tris-HCl pH8.5, 30% PEG 3350