8JVW
Crystal structure of the dimeric DZBB fold protein tkoL2_v1.2_Z
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-10-12 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 23.959, 44.427, 84.289 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.145 - 1.806 |
R-factor | 0.2128 |
Rwork | 0.211 |
R-free | 0.23270 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.024 |
RMSD bond angle | 1.855 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Number of reflections | 7929 | 1257 |
<I/σ(I)> | 18.77 | |
Completeness [%] | 99.9 | |
Redundancy | 3.4 | |
CC(1/2) | 0.990 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 70% MPD, 100mM HEPES pH7.0 |