8JVT
Crystal structure of the chimeric protein mkaL2_v1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2022-07-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 124.137, 53.887, 55.654 |
Unit cell angles | 90.00, 113.41, 90.00 |
Refinement procedure
Resolution | 38.684 - 2.106 |
R-factor | 0.2069 |
Rwork | 0.202 |
R-free | 0.24840 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.267 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Number of reflections | 35872 | 5832 |
<I/σ(I)> | 0.78 | |
Completeness [%] | 93.3 | |
Redundancy | 1.82 | |
CC(1/2) | 0.990 | 0.440 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 6000, 100mM Tris pH8.0, 200mM Lithium chrolide |