8JVR
Crystal structure of the dimeric RIFT fold protein Ph1_GG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 34.449, 34.449, 55.085 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.834 - 1.794 |
R-factor | 0.2388 |
Rwork | 0.238 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.698 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.900 |
High resolution limit [Å] | 1.794 | 1.794 |
Number of reflections | 6734 | 2934 |
<I/σ(I)> | 12.5 | |
Completeness [%] | 98.2 | |
Redundancy | 2.9 | |
CC(1/2) | 0.980 | 0.920 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 50% PEG400, 100mM Sodium acetate/Acetic acid pH4.5, 200mM Lithium sulfate |