8JVQ

Crystal structure of the dimeric RIFT fold protein Ph1_DG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2022-04-22
Detector	RAYONIX MX-225
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	35.017, 35.017, 54.660
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.518 - 1.401
R-factor	0.2135
Rwork	0.212
R-free	0.24090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.017
RMSD bond angle	1.280
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.500
High resolution limit [Å]	1.400	1.400
Number of reflections	14659	2362
<I/σ(I)>	12.45
Completeness [%]	99.2
Redundancy	11.1
CC(1/2)	1.000	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	3200mM Ammonium sulfate, 100mM Citric acid/Sodium hydroxide pH5.0