8JVP

Crystal structure of the dimeric DZBB fold protein Ph1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2022-01-27
Detector	RAYONIX MX-225
Wavelength(s)	1
Spacegroup name	P 32 2 1
Unit cell lengths	35.056, 35.056, 54.844
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.350 - 1.302
R-factor	0.1933
Rwork	0.190
R-free	0.22510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.205
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.380
High resolution limit [Å]	1.300	1.300
Number of reflections	18226	31946
<I/σ(I)>	0.42
Completeness [%]	98.7
Redundancy	18.2
CC(1/2)	1.000	0.990

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	30% PEG 400, 100mM Tris pH8.0, 400mM Lithium sulfate