8JV0

Crystal structure of the SLA-2*1001 allele and ASFV antigenic peptide at 2.2A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-20
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	P 21 21 21
Unit cell lengths	41.944, 105.317, 221.350
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.030 - 2.200
R-factor	0.22319
Rwork	0.222
R-free	0.24882
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.636
Data reduction software	HKL-3000 (7.21)
Data scaling software	HKL-3000 (7.21)
Phasing software	PHASER (2.7.0)
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.940	1.940
Rpim	0.027
Number of reflections	39759	3586
<I/σ(I)>	6	0.25
Completeness [%]	98.4
Redundancy	9.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Lithium sulfate monohydrate,0.1 M TRIS hydrochloride pH 8.5,30% w/v Polyethylene glycol 4,000