8JUH
Crystal structure of ScFv against the receptor binding domine of SARS-CoV-2 S-spike protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-01-01 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 60.210, 77.753, 61.346 |
Unit cell angles | 90.00, 112.25, 90.00 |
Refinement procedure
Resolution | 55.790 - 2.210 |
R-factor | 0.25953 |
Rwork | 0.257 |
R-free | 0.30959 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.008 |
RMSD bond angle | 1.595 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 77.750 | 2.270 |
High resolution limit [Å] | 2.210 | 2.210 |
Rmerge | 0.693 | 2.980 |
Rmeas | 0.758 | 3.273 |
Rpim | 0.301 | 1.327 |
Total number of observations | 172092 | 12384 |
Number of reflections | 26361 | 1947 |
<I/σ(I)> | 4.1 | 1.6 |
Completeness [%] | 100.0 | |
Redundancy | 6.5 | 6.4 |
CC(1/2) | 0.708 | 0.118 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289.15 | 25% Polyethylene glycol 3350,0.1 M HEPES,PH7.5 |