8JU3
Mu phage tail fiber
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-10-27 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 63.839, 63.839, 462.383 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.880 - 2.000 |
| R-factor | 0.1984 |
| Rwork | 0.198 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.357 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.880 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 76418 | 3548 |
| <I/σ(I)> | 8.4 | |
| Completeness [%] | 99.8 | |
| Redundancy | 9.39 | |
| CC(1/2) | 0.996 | 0.611 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M HEPES (pH 7.5), 4.3M NaCl |
