8JTL
Structure of OY phytoplasma SAP05 binding with AtRpn10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL10U2 |
| Synchrotron site | SSRF |
| Beamline | BL10U2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-23 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.979176 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.410, 39.370, 121.940 |
| Unit cell angles | 90.00, 93.79, 90.00 |
Refinement procedure
| Resolution | 25.350 - 1.780 |
| R-factor | 0.1857 |
| Rwork | 0.184 |
| R-free | 0.21850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.356 |
| Data reduction software | xia2 |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | PHENIX (1.19.2_4158) |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.080 | 1.730 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Rmerge | 0.169 | 1.773 |
| Rmeas | 0.185 | |
| Rpim | 0.074 | 0.763 |
| Number of reflections | 55704 | 4021 |
| <I/σ(I)> | 5 | 1 |
| Completeness [%] | 99.2 | 98.6 |
| Redundancy | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 15% w/v PEG 6000, 5% v/v MPD, 0.1M MES pH 6.5 |
