8JTK

Structure of AYWB phytoplasma SAP05 recognizing AtRpn10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL10U2
Synchrotron site	SSRF
Beamline	BL10U2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-17
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.979183
Spacegroup name	P 32 2 1
Unit cell lengths	49.240, 49.240, 215.154
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.930 - 1.570
R-factor	0.1883
Rwork	0.187
R-free	0.21540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.033
Data scaling software	Aimless (0.7.7)
Phasing software	PHENIX (1.19.2_4158)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.720	1.650
High resolution limit [Å]	1.570	1.570
Rmerge	0.056	0.768
Rmeas	0.060	0.831
Rpim	0.021	0.314
Number of reflections	42242	6330
<I/σ(I)>	18	2.6
Completeness [%]	95.9	100
Redundancy	8.2
CC(1/2)	0.999	0.736

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Sodium malonate pH 5.0, 20% w/v Polyethylene glycol 3,350