8JSZ
Crystal structure of a uridylate cyclase from Anabaena sp.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSRRC BEAMLINE TPS 07A |
Synchrotron site | NSRRC |
Beamline | TPS 07A |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-21 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97626 |
Spacegroup name | P 1 |
Unit cell lengths | 47.661, 50.792, 71.391 |
Unit cell angles | 71.21, 83.96, 62.07 |
Refinement procedure
Resolution | 25.050 - 1.830 |
R-factor | 0.17765 |
Rwork | 0.177 |
R-free | 0.17893 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | AlphaFold |
RMSD bond length | 0.014 |
RMSD bond angle | 1.731 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.910 |
High resolution limit [Å] | 1.830 | 1.840 |
Number of reflections | 47377 | 4637 |
<I/σ(I)> | 27.5 | |
Completeness [%] | 97.6 | 95.1 |
Redundancy | 5.5 | 5.3 |
CC(1/2) | 0.998 | 0.972 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M BIS-TRIS pH 6.5, 0.2 M Magnesium chloride hexahydrate, 25% w/v Polyethylene glycol 3,350 |