8JRI
Cryo-EM structure of human 26S proteasomal RP subcomplex (Ea state) without any bound substrate.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.4 |
| Number of Particle | 2695911 |
| Software | Topaz,PHENIX,Coot,RELION,cryoSPARC,EPU,CTFFIND,UCSF ChimeraX |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
