8JRB
Structure of DNA polymerase 1 from Aquifex pyrophilus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE MASSIF-1 |
Synchrotron site | ESRF |
Beamline | MASSIF-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-02-06 |
Detector | DECTRIS PILATUS 2M |
Wavelength(s) | 0.969 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 178.826, 74.725, 129.560 |
Unit cell angles | 90.00, 108.25, 90.00 |
Refinement procedure
Resolution | 46.260 - 2.200 |
R-factor | 0.193 |
Rwork | 0.191 |
R-free | 0.23290 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.261 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.910 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Rpim | 0.031 | 0.505 |
Number of reflections | 80316 | 4294 |
<I/σ(I)> | 15.5 | |
Completeness [%] | 97.5 | 96.8 |
Redundancy | 4.7 | 4.9 |
CC(1/2) | 0.994 | 0.669 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 18% PEG 8K, 100 mM Ammonium Sulphate |