8JR5

Crystal structure of Hendra Virus attachment(G) glycoprotein mutant S586N

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-17
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.9789
Spacegroup name	P 1 21 1
Unit cell lengths	83.926, 63.658, 121.622
Unit cell angles	90.00, 97.69, 90.00

Refinement procedure

Resolution	41.590 - 3.300
R-factor	0.2599
Rwork	0.258
R-free	0.29000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.589
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.418
High resolution limit [Å]	3.300	3.300
Number of reflections	19331	19216
<I/σ(I)>	12.68
Completeness [%]	98.5
Redundancy	6.2
CC(1/2)	0.980	0.980

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		289	PEG3350 14%, 10mM MgCl2, 5mM NiCL2, PIPES 6.5