8JR4
Molecular structure of bat MHC II at 2.4 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.987 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 92.160, 92.160, 107.365 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.430 - 2.300 |
R-factor | 0.21457 |
Rwork | 0.212 |
R-free | 0.26309 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.542 |
Data reduction software | HKL-2000 (7.21) |
Data scaling software | HKL-3000 (7.21) |
Phasing software | PHASER (2.7.0) |
Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.180 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.189 | 1.073 |
Number of reflections | 22821 | 2453 |
<I/σ(I)> | 15.667 | |
Completeness [%] | 100.0 | |
Redundancy | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 0.2 M Calcium acetate hydrate,20% w/v Polyethylene glycol 3,350,pH7.5 |