8JQZ
Crystal Structure of GppNHp-bound mIRGB10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-11-29 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.640, 62.440, 119.230 |
| Unit cell angles | 90.00, 99.56, 90.00 |
Refinement procedure
| Resolution | 29.430 - 3.050 |
| R-factor | 0.2337 |
| Rwork | 0.232 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.915 |
| Data reduction software | XDS |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.430 | 3.159 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Number of reflections | 17524 | 1743 |
| <I/σ(I)> | 12.86 | |
| Completeness [%] | 99.4 | 99.43 |
| Redundancy | 6.6 | 6.8 |
| CC(1/2) | 0.998 | 0.866 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% (w/v) PEG 2,000 monomethyl ether (MME), 0.2 M trimethylamine n-oxide, 0.1 M Tris-HCl (pH 8.5) |
