8JQ0

Crystal structure of ZBTB48 ZF10-11-C in complex with CIITA promoter

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9785
Spacegroup name	C 1 2 1
Unit cell lengths	126.563, 46.864, 79.047
Unit cell angles	90.00, 124.89, 90.00

Refinement procedure

Resolution	39.440 - 2.900
R-factor	0.2361
Rwork	0.233
R-free	0.29120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5yj3
RMSD bond length	0.003
RMSD bond angle	0.473
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	2.950
High resolution limit [Å]	2.900	7.850	2.900
Rmerge	0.117	0.069	0.867
Rmeas	0.128	0.076	0.954
Rpim	0.050	0.031	0.388
Total number of observations	52860
Number of reflections	8503	455	406
<I/σ(I)>	4.9
Completeness [%]	98.9	99.6	91.4
Redundancy	6.2	6.1	5
CC(1/2)	0.989	0.992	0.815

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M Ammonium acetate, 0.1 M bis-Tris pH 5.5, 25% w/v PEG 3350