8JOY

Plk1 polo-box domain bound to HPV4 L2 residues 251-257 with pThr255

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	93
Detector technology	PIXEL
Collection date	2023-05-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	35.106, 54.691, 57.578
Unit cell angles	90.00, 90.02, 90.00

Refinement procedure

Resolution	39.650 - 2.610
R-factor	0.2034
Rwork	0.198
R-free	0.25460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.179
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.112	0.503
Number of reflections	6436	315
<I/σ(I)>	5.19
Completeness [%]	95.5
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M malate MES Tris buffer pH 6.5, 25% polyethylene glycol 1500