8JOR
Structure of an acyltransferase involved in mannosylerythritol lipid formation from Pseudozyma tsukubaensis in type A crystal
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-10-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.043, 83.073, 131.035 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.080 - 1.450 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.19430 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.897 |
Data reduction software | XDS (Jan 10, 2022) |
Data scaling software | XDS (Jan 10, 2022) |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 44.730 | 44.730 | 1.530 |
High resolution limit [Å] | 1.450 | 4.320 | 1.450 |
Rmerge | 0.075 | 0.033 | 1.177 |
Rmeas | 0.081 | 0.035 | 1.262 |
Rpim | 0.030 | ||
Number of reflections | 103745 | 4183 | 16481 |
<I/σ(I)> | 15.68 | 2.29 | |
Completeness [%] | 99.8 | 99.6 | 99.2 |
Redundancy | 7.4 | ||
CC(1/2) | 0.999 | 0.999 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 21% PEG 3350, 300mM lithium chloride |