8JOQ

Plk1 polo-box domain bound to HPV18 L2 residues 209-215 with pThr213

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	93
Detector technology	CCD
Collection date	2023-03-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.987
Spacegroup name	P 1 21 1
Unit cell lengths	35.143, 54.346, 57.295
Unit cell angles	90.00, 90.61, 90.00

Refinement procedure

Resolution	35.141 - 1.796
R-factor	0.21
Rwork	0.206
R-free	0.24970
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.891
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.796	1.800
Number of reflections	18536	958
<I/σ(I)>	5.5
Completeness [%]	91.8
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.1M malate MES Tris buffer pH 6.5, 25% polyethylene glycol 1500