8JOH
structure of Echinococcus multilocularis Cystatin B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-09-19 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.720, 65.990, 92.710 |
| Unit cell angles | 90.00, 103.38, 90.00 |
Refinement procedure
| Resolution | 53.314 - 2.040 |
| Rwork | 0.250 |
| R-free | 0.28160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.666 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.314 | 2.090 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 54430 | 4003 |
| <I/σ(I)> | 11.6 | |
| Completeness [%] | 99.6 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.997 | 0.758 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 30% PEG400 100 mM Tris base/ Hydrochloric acid pH 8.5 200 mM Magnesium chloride |
