8JNX
alkaline amylase Amy703 with truncated of N-terminus domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-25 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97890 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 171.307, 171.307, 167.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.458 - 3.203 |
| R-factor | 0.209187704736 |
| Rwork | 0.207 |
| R-free | 0.24457 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.483 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.260 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.079 | |
| Number of reflections | 24396 | 1955 |
| <I/σ(I)> | 35 | |
| Completeness [%] | 98.1 | |
| Redundancy | 13.7 | |
| CC(1/2) | 0.779 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 0.1 M Tris-HCl, pH 7.5, 2.4 M Sodium malonate dibasic |
