8JNU
Crystal structure of V30G mutated human transthyretin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-10-24 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 43.100, 85.400, 64.480 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 35.600 - 1.640 |
R-factor | 0.1863 |
Rwork | 0.185 |
R-free | 0.20680 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.883 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.460 | 1.730 |
High resolution limit [Å] | 1.640 | 1.640 |
Rmerge | 0.294 | |
Number of reflections | 29770 | 4265 |
<I/σ(I)> | 21.5 | |
Completeness [%] | 99.7 | |
Redundancy | 6.9 | |
CC(1/2) | 0.999 | 0.939 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 293 | PEG400, MgCl2, glycerol |