8JNT
RxLR effector RxLR19 from Phytophthora capsici
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL18U1 |
Synchrotron site | SSRF |
Beamline | BL18U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-05-23 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.979 |
Spacegroup name | P 61 |
Unit cell lengths | 49.741, 49.741, 61.170 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.077 - 2.201 |
R-factor | 0.175 |
Rwork | 0.174 |
R-free | 0.20300 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.058 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | SOLVE |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 43.077 |
High resolution limit [Å] | 2.200 | 2.201 |
Rmerge | 0.157 | 0.901 |
Number of reflections | 4403 | 4403 |
<I/σ(I)> | 17.75 | |
Completeness [%] | 100.0 | |
Redundancy | 20 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 283 | 0.03M Citric acid, 0.07M BIS-TRIS propane pH 7.6, 20% w/v PEG 3350 |