8JMY
Crystal structure of Rv2186c in Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 80 |
| Detector technology | CCD |
| Collection date | 2021-05-19 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.477, 28.254, 86.779 |
| Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
| Resolution | 27.291 - 1.563 |
| Rwork | 0.158 |
| R-free | 0.20380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.633 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0411) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.590 |
| High resolution limit [Å] | 1.560 | 1.560 |
| Rmeas | 0.074 | 0.329 |
| Rpim | 0.029 | 0.138 |
| Number of reflections | 36125 | 712 |
| <I/σ(I)> | 27.8 | |
| Completeness [%] | 95.9 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.980 | 0.949 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 289 | 0.1M Tris-HCl, pH7.1, 0.3M MgCl2, 10% PEG 8000 |
