8JMK

Structure of Helicobacter pylori Soj mutant, D41A bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2022-12-07
Detector	RAYONIX MX300-HS
Wavelength(s)	0.99984
Spacegroup name	P 1
Unit cell lengths	75.200, 75.198, 81.124
Unit cell angles	71.25, 71.38, 67.70

Refinement procedure

Resolution	27.879 - 2.700
R-factor	0.2276
Rwork	0.224
R-free	0.30340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iuc
RMSD bond length	0.010
RMSD bond angle	1.619
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.690
High resolution limit [Å]	2.600	5.590	2.600
Rmerge	0.042	0.021	0.337
Rmeas		0.030	0.477
Rpim		0.021	0.337
Number of reflections	44627	4505	4466
<I/σ(I)>	10.7
Completeness [%]	96.4	97.3	96.6
Redundancy	1.9	1.9	1.9
CC(1/2)	0.756	0.998	0.756

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	25% Ethylene glycol