8JMJ

Structure of Helicobacter pylori Soj-DNA-Spo0J complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE TPS 05A
Synchrotron site	NSRRC
Beamline	TPS 05A
Temperature [K]	100
Detector technology	CCD
Collection date	2022-12-07
Detector	RAYONIX MX300-HS
Wavelength(s)	0.99984
Spacegroup name	P 1
Unit cell lengths	75.178, 75.193, 81.116
Unit cell angles	71.34, 71.46, 67.70

Refinement procedure

Resolution	28.565 - 2.570
R-factor	0.2275
Rwork	0.224
R-free	0.30170
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iuc
RMSD bond length	0.011
RMSD bond angle	1.774
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	2.660
High resolution limit [Å]	2.570	5.530	2.570
Rmerge	0.039	0.020	0.321
Rmeas		0.028	0.455
Rpim		0.020	0.321
Number of reflections	46674	4711	4658
<I/σ(I)>	11.1
Completeness [%]	96.5	97.4	96.2
Redundancy	1.9	2	1.8
CC(1/2)		0.998	0.778

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	0.1 M MES and 25% Ethylene glycol