8JLT

Crystal structure of the Green fluorescent protein SET203EF223DA227 variant at pH 7.0

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 5C (4A)
Synchrotron site	PAL/PLS
Beamline	5C (4A)
Temperature [K]	193
Detector technology	CCD
Collection date	2022-06-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	38.184, 122.875, 58.696
Unit cell angles	90.00, 101.06, 90.00

Refinement procedure

Resolution	33.380 - 1.940
R-factor	0.2028
Rwork	0.201
R-free	0.22870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.186
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	4.700	2.020
High resolution limit [Å]	1.940	4.200	1.950
Rmerge	0.079	0.054	0.225
Rmeas	0.087	0.058	0.267
Rpim	0.035	0.022	0.139
Number of reflections	36088	3955	2580
<I/σ(I)>	9.2
Completeness [%]	91.9	99.4	65.2
Redundancy	4.5	6.6	2.6
CC(1/2)	0.994	0.998	0.880

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M Imidazole pH 7.0, 40% (w/v) Isopropanol, 15% (w/v) PEG 8000