8JJT

Structure of SenB in complex with UDP-GlcNAc at 1.88 Angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	108.223, 56.375, 108.445
Unit cell angles	90.00, 91.11, 90.00

Refinement procedure

Resolution	48.848 - 1.880
Rwork	0.186
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.818
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.850	1.910
High resolution limit [Å]	1.880	1.880
Number of reflections	106627	5198
<I/σ(I)>	15.2
Completeness [%]	99.8
Redundancy	6.6
CC(1/2)	0.998	0.771

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.153	15% PEG 20000, 0.1M Hepes/NaOH pH 7.0