8JJB

Crystal structure of T2R-TTL-Y61 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	105.287, 157.915, 181.748
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.060 - 2.680
R-factor	0.2272
Rwork	0.227
R-free	0.26010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.572
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.470	2.790
High resolution limit [Å]	2.680	2.680
Number of reflections	934816	32495
<I/σ(I)>	1.44
Completeness [%]	99.0
Redundancy	11.2
CC(1/2)	0.890	0.540

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	6% poly(ethylene glycol) 4000, 8% glycerol, 0.1 M MES (pH 6.7), 30 mM CaCl2, 30 mM MgCl2