8JI9
Crystal Structure of cas7 and AcrIE10 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL02U1 |
Synchrotron site | SSRF |
Beamline | BL02U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-08-25 |
Detector | DECTRIS EIGER2 S 9M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 151.770, 46.240, 133.500 |
Unit cell angles | 90.00, 101.29, 90.00 |
Refinement procedure
Resolution | 43.640 - 2.540 |
R-factor | 0.2389 |
Rwork | 0.238 |
R-free | 0.25440 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.254 |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.640 | 2.610 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmerge | 0.122 | |
Rmeas | 0.133 | |
Rpim | 0.052 | |
Number of reflections | 30500 | 15719 |
<I/σ(I)> | 11.5 | |
Completeness [%] | 99.8 | 99.8 |
Redundancy | 6.7 | |
CC(1/2) | 0.997 | 0.699 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 300 | 4% v/v TacsimateTM pH 7.0, 12% w/v Polyethylene glycol 3,350 |