8JI1

Crystal structure of Ham1 from Plasmodium falciparum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SEALED TUBE
Source details	BRUKER IMUS 3.0 MICROFOCUS
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-01-02
Detector	Bruker PHOTON III
Wavelength(s)	1.53
Spacegroup name	P 21 21 21
Unit cell lengths	63.294, 73.658, 111.989
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.170 - 2.400
R-factor	0.1986
Rwork	0.195
R-free	0.25650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4f95
RMSD bond length	0.007
RMSD bond angle	0.896
Data reduction software	PROTEUM PLUS
Data scaling software	PROTEUM PLUS
Phasing software	PHASER
Refinement software	PHENIX ((1.14_3260: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.080	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	20443	2080
<I/σ(I)>	20.7
Completeness [%]	100.0
Redundancy	12.4
CC(1/2)	0.998	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	16% PEG 4000, 100 mM Citrate buffer pH 5.5, 20% Isopropanol