8JHV

The first crystal structure of a H-2Kb-restricted decapeptide from Cryptosporidium parvum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2021-11-20
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97915
Spacegroup name	P 32 1 2
Unit cell lengths	89.380, 89.380, 296.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.105 - 3.470
Rwork	0.230
R-free	0.29678
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.160	3.800
High resolution limit [Å]	3.470	3.470
Number of reflections	18018	4242
<I/σ(I)>	11.3
Completeness [%]	99.9
Redundancy	20
CC(1/2)	0.999	0.875

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20%(w/v) Polyethylene glycol 3350; 0.2M Lithium acetate dihydrate