8JFU
AcrIIA15 in complex with palindromic DNA substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-12-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97851 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 110.604, 131.388, 234.183 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.130 - 3.150 |
R-factor | 0.2599 |
Rwork | 0.259 |
R-free | 0.27480 |
Structure solution method | SAD |
RMSD bond length | 0.008 |
RMSD bond angle | 1.111 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.220 |
High resolution limit [Å] | 3.150 | 3.150 |
Number of reflections | 30070 | 1942 |
<I/σ(I)> | 10 | |
Completeness [%] | 99.7 | |
Redundancy | 5.5 | |
CC(1/2) | 0.999 | 0.604 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 0.2 M Sodium chloride, 0.1 M HEPES-NaOH pH 7.5, 25% w/v PEG 3,350 |