8JFR
N-terminal domain of AcrIIA15 in complex with palindromic DNA substrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2023-03-26 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97851 |
Spacegroup name | P 43 |
Unit cell lengths | 58.712, 58.712, 240.593 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.660 - 3.100 |
R-factor | 0.2468 |
Rwork | 0.246 |
R-free | 0.25890 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.293 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 3.100 |
Number of reflections | 14903 | 725 |
<I/σ(I)> | 24.9 | |
Completeness [%] | 99.9 | |
Redundancy | 4.7 | |
CC(1/2) | 1.000 | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.6 M sodium citrate pH 6.05 |