8JFO

Crystal structure of anti-CRISPR protein AcrIIA15

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-08-28
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	P 41
Unit cell lengths	88.194, 88.194, 145.649
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.680 - 2.300
R-factor	0.2225
Rwork	0.222
R-free	0.23270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	previously solved AcrIIA15(CTD) structure using SAD
RMSD bond length	0.010
RMSD bond angle	1.307
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.680	2.340
High resolution limit [Å]	2.300	2.300
Number of reflections	49185	2431
<I/σ(I)>	21.6
Completeness [%]	99.7
Redundancy	5.8
CC(1/2)	0.996	0.645

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.2 M Trimethylamine N-oxide dihydrate, 0.1 M Tris pH 8.82, 18% w/v PEG 2,000