8JEA

Crystal structure of CGL1 from Crassostrea gigas, mannotriose-bound form (CGL1/Man(alpha)1-2Man(alpha)1-2Man)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	90
Detector technology	PIXEL
Collection date	2021-12-02
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1.0
Spacegroup name	P 1 21 1
Unit cell lengths	39.734, 58.674, 108.410
Unit cell angles	90.00, 93.66, 90.00

Refinement procedure

Resolution	39.771 - 0.970
Rwork	0.175
R-free	0.18850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.014
RMSD bond angle	1.799
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.771	0.990
High resolution limit [Å]	0.970	0.970
Rmerge	0.036	1.021
Number of reflections	292966	14427
<I/σ(I)>	28.6
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)	1.000	0.820

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.2 M magnesium acetate, 0.1 M sodium cacodylate (pH 6.5), 20% PEG 8000