8JE9
Crystal structure of CGL1 from Crassostrea gigas, mannobiose-bound form (CGL1/Man(alpha)1-2Man)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2021-12-02 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.749, 58.701, 108.405 |
| Unit cell angles | 90.00, 93.70, 90.00 |
Refinement procedure
| Resolution | 39.778 - 1.000 |
| Rwork | 0.174 |
| R-free | 0.18690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.768 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.800 | 1.020 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.056 | |
| Number of reflections | 229413 | 4020 |
| <I/σ(I)> | 18.6 | |
| Completeness [%] | 85.9 | |
| Redundancy | 6.8 | |
| CC(1/2) | 0.999 | 0.872 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 0.2 M magnesium acetate, 0.1 M sodium cacodylate (pH 6.5), 20% (w/v) PEG 8000 |
