8JDI

Crystal structure of Cas7-AcrIF25 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97919
Spacegroup name	P 21 21 21
Unit cell lengths	108.666, 119.008, 215.991
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.426 - 3.372
R-factor	0.2193
Rwork	0.218
R-free	0.24690
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6b45 7WXV
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.480
High resolution limit [Å]	3.370	3.400
Number of reflections	40193	2587
<I/σ(I)>	8.4
Completeness [%]	99.4
Redundancy	5.4
CC(1/2)	0.981	0.372

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		289	0.1 M Bis-tris propane, pH 6.5, 21 % PEG3350, 0.2 M Lithium acetate