8JCA

Cyrstal structure of SKIP RUN domain in complex with GTP-bound Arl8b(Q75L)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL02U1
Synchrotron site	SSRF
Beamline	BL02U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-20
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	178.687, 53.836, 40.512
Unit cell angles	90.00, 98.13, 90.00

Refinement procedure

Resolution	32.050 - 1.650
R-factor	0.1761
Rwork	0.175
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.034
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX ((1.17.1_3660: ???))
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	51.504	1.680
High resolution limit [Å]	1.647	1.650
Rmerge		0.775
Number of reflections	41074	41074
<I/σ(I)>	0.073
Completeness [%]	89.1
Redundancy	2.7
CC(1/2)	0.990	0.400

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		298	800mM Potassiam phosphate dibasic 1.2 M Sodium phosphate monobasic 0.1M CAPS/Sodium hydroxide 0.2M Lithium sulfate pH10.5