8JBI
SteC 202-375 mutant- C276S
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL02U1 |
| Synchrotron site | SSRF |
| Beamline | BL02U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-02 |
| Detector | DECTRIS EIGER2 S 9M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.998, 92.880, 88.838 |
| Unit cell angles | 90.00, 107.82, 90.00 |
Refinement procedure
| Resolution | 40.398 - 2.356 |
| R-factor | 0.1972 |
| Rwork | 0.195 |
| R-free | 0.24220 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.163 |
| Data scaling software | STARANISO (1.0.5) |
| Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.398 | 2.480 |
| High resolution limit [Å] | 2.356 | 2.360 |
| Rmerge | 0.072 | 0.680 |
| Rmeas | 0.724 | |
| Rpim | 0.022 | 0.246 |
| Number of reflections | 35432 | 5280 |
| <I/σ(I)> | 24.3 | 2.9 |
| Completeness [%] | 96.9 | 99.1 |
| Redundancy | 11.4 | |
| CC(1/2) | 0.896 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 291.15 | Sodium cacodylate trihydrate, Ammonium sulfate, Magnesium sulfate heptahydrate |
